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KMID : 1036320130350030143
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Maxillofacial Plastic and Reconstructive Surgery
2013 Volume.35 No. 3 p.143 ~ p.154
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Induction of Selective Cell Death of Oral Squamous Carcinoma Cells by Integrin α2 Antibody and EGFR Antibody
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Choi Yeon-Sik
Hwang Dae-Seok
Shin Sang-Hun
Kim Uk-Kyu
Kim Gyoo-Cheon
Yoon Sik
Kim Chul-Hoon
Jeon Yeong-Chan
Byun June-Ho
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Abstract
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Purpose: This study was to find efficacy of integrin alpha2 (${\alpha}_2$ 수식 이미지) and epidermal growth factor receptor (EGFR) as tumor marker of oral squamous cell carcinoma (SCC) and clarify the selective cell death effect of anti-integrin ${\alpha}_2$ 수식 이미지 and anti-EGFR on SCC cells, additionally testify conjugated gold nanoparticles (GNP) with air plasma for selective cell death of oral SCC.
Methods: Expression of integrin ${\alpha}_2$ 수식 이미지, EGFR on human SCC cells (SCC25) were examined by western blot. SCC25 cells were treated with anti-integrin ${\alpha}_2$ 수식 이미지, anti-EGFR and analysed by Hemacolor staining, immunoflorescence staining, FACS flow cytometry. Conjugated GNP with integrin ${\alpha}_2$ 수식 이미지, EGFR antibody were treated by air plasma on SCC cells.
Results: Integrin ${\alpha}_2$ 수식 이미지 and EGFR were over-expressed on SCC25 cells than normal lung WI-38 cells. The cell viability rate of SCC25 cells treated with anti-integrin ${\alpha}_2$ 수식 이미지, anti-EGFR was lower than WI-38 cells. The concentration changes of nucleus, releasing cytochrome c and apoptosis inducing factor (AIF) from mitochondria to cytosol were observed. The changes of proteins related with apoptosis were observed. Increase of bax, bcl-xL, activation of caspase-3, -7, -9, and fragmentation of PARP, DFF45 and decrease of lamin A/C in SCC25 cells were observed. In FACS, increase of sub-$G_1$ 수식 이미지 and S phase was observed. Cell cycle related proteins, Such as cyclin D1, cyclin dependent kinase (CDK) 4, cyclin A, cyclin E, CDK 2, p27 were decreased. After SCC25 cells treated with conjugatged GNP-Integrin ${\alpha}_2$ 수식 이미지, GNP-EGFR, additionally air plasma, the cell death rate was significantly increased.
Conclusion: Integrin ${\alpha}_2$ 수식 이미지, EGFR were over-expressed in oral SCC cells. Anti-integrin ${\alpha}_2$ 수식 이미지, anti-EGFR in SCC25 cells induced apoptosis selectively. When GNP-anti integrin ${\alpha}_2$ 수식 이미지, GNP-anti EGFR were treated with air plasma on SCC25 cells, cancer cells were died more selectively. GNP-anti integrin ${\alpha}_2$ 수식 이미지, GNP-anti EGFR with air plasma could be treatment choice of oral SCC.
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KEYWORD
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OSCC, Integrin alpha2, EGFR, Gold nanoparticles, Cell death
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